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Evaluation of fecal culture and fecal RT-PCR to detect Mycobacterium avium ssp. paratuberculosis fecal shedding in dairy goats and dairy sheep using latent class Bayesian modeling

By Cathy A. Bauman, Andria Jones-Bitton, Jocelyn Jansen, David Kelton, and Paula Menzies; BioMed Central

The study’s objective was to evaluate the ability of fecal culture (FCUL) and fecal PCR (FPCR) to identify dairy goat and dairy sheep shedding Mycobacterium avium ssp. paratuberculosis. A cross-sectional study of the small ruminant populations was performed in Ontario, Canada between October 2010 and August 2011. Twenty-nine dairy goat herds and 21 dairy sheep flocks were visited, and 20 lactating females > two years of age were randomly selected from each farm resulting in 580 goats and 397 sheep participating in the study. Feces were collected per rectum and cultured using the BD BACTEC™ MGIT™ 960 system using a standard (49 days) and an extended (240 days) incubation time, and underwent RT-PCR based on the hsp-X gene (Tetracore®). Statistical analysis was performed using a 2-test latent class Bayesian hierarchical model for each species fitted in WinBUGS.

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Vaccination plays important role in footrot control

By Irish Farmers Journal

Vaccination is an integral component of the footrot control programme for leading Texel breeder Michael McHugh. “We have been vaccinating for the past six years and we find that, when combined with effective treatment, proper foot bathing and culling of chronic cases, it has given excellent results,” said Michael, who runs a flock of 300 ewes at Lisnashannagh on the outskirts of Carrickmacross in Monaghan.

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